Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging

Angew Chem Int Ed Engl. 2016 Jul 25;55(31):8948-52. doi: 10.1002/anie.201603281. Epub 2016 Jun 13.

Abstract

We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.

Keywords: DNA; hydrogels; photochemistry; two-photon uncaging; wavelength selectivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Color*
  • DNA / chemistry*
  • Nucleic Acid Hybridization*
  • Photons*

Substances

  • DNA