Evaluation of four commonly used normalizer genes for the study of decidual gene expression

Placenta. 2016 Jul:43:9-12. doi: 10.1016/j.placenta.2016.04.009. Epub 2016 Apr 26.

Abstract

Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers (RNA18S, GAPDH, TBP, and YWHAZ) across 59 decidual tissue samples collected by vacuum suction from preeclamptic and normotensive pregnancies. RNA18S and GAPDH were not suitable as normalizers, while YWHAZ and TBP were stably expressed across the study groups.

Keywords: Decidua basalis; Gene expression; Housekeeping gene; Normalizer gene; Preeclampsia; Quantitative PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / genetics
  • 14-3-3 Proteins / metabolism
  • Decidua / metabolism*
  • Female
  • Gene Expression Profiling / methods
  • Gene Expression*
  • Genes, Essential / genetics*
  • Humans
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • RNA, Ribosomal, 18S / genetics
  • RNA, Ribosomal, 18S / metabolism
  • Real-Time Polymerase Chain Reaction / methods*
  • TATA-Box Binding Protein / genetics
  • TATA-Box Binding Protein / metabolism

Substances

  • 14-3-3 Proteins
  • RNA, Messenger
  • RNA, Ribosomal, 18S
  • TATA-Box Binding Protein
  • TBP protein, human
  • YWHAZ protein, human