Glutathione, glutathione-related enzymes, and oxidative stress in individuals with subacute occupational exposure to lead

Environ Toxicol Pharmacol. 2016 Jul:45:235-40. doi: 10.1016/j.etap.2016.06.008. Epub 2016 Jun 7.

Abstract

The aim of the study was to investigate the influence of subacute exposure to lead on the glutathione-related antioxidant defense and oxidative stress parameters in 36 males occupationally exposed to lead for 40±3.2days. Blood lead level in the examined population increased significantly by 359% due to lead exposure. Simultaneously, erythrocyte glutathione level decreased by 16%, whereas the activity of glutathione-6-phosphate dehydrogenase in erythrocytes and leukocytes decreased by 28% and 10%, respectively. Similarly, the activity of glutathione-S-transferase in erythrocytes decreased by 45%. However, the activity of glutathione reductase in erythrocytes and leukocytes increased by 26% and 6%, respectively, whereas the total oxidant status value in leukocytes increased by 37%. Subacute exposure to lead results in glutathione pool depletion and accumulation of lipid peroxidation products; however, it does not cause DNA damage. Besides, subacute exposure to lead modifies the activity of glutathione-related enzymes.

Keywords: DNA damage; Glutathione; Lipid peroxidation; Oxidative stress; Subacute exposure to lead.

MeSH terms

  • Adult
  • Antioxidants / metabolism
  • Erythrocytes / drug effects
  • Erythrocytes / enzymology
  • Glucosephosphate Dehydrogenase / metabolism
  • Glutathione / metabolism*
  • Glutathione Peroxidase / metabolism*
  • Glutathione Reductase / metabolism*
  • Glutathione Transferase / metabolism*
  • Humans
  • Lead / blood
  • Lead / toxicity*
  • Leukocytes / drug effects
  • Leukocytes / enzymology
  • Lipid Peroxidation / drug effects
  • Male
  • Middle Aged
  • Occupational Exposure / adverse effects*
  • Occupational Exposure / analysis
  • Oxidative Stress / drug effects*
  • Poland
  • Time Factors
  • Young Adult

Substances

  • Antioxidants
  • Lead
  • Glucosephosphate Dehydrogenase
  • Glutathione Peroxidase
  • Glutathione Reductase
  • Glutathione Transferase
  • Glutathione