Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host

EBioMedicine. 2016 Jul:9:161-169. doi: 10.1016/j.ebiom.2016.05.037. Epub 2016 May 28.

Abstract

Bacteria in humans play an important role in health and disease. Considerable emphasis has been placed in understanding the role of bacteria in host-microbiome interkingdom communication. Here we show that serotonin, responsible for mood in the brain and motility in the gut, can also act as a bacterial signaling molecule for pathogenic bacteria. Specifically, we found that serotonin acts as an interkingdom signaling molecule via quorum sensing and that it stimulates the production of bacterial virulence factors and increases biofilm formation in vitro and in vivo in a novel mouse infection model. This discovery points out at roles of serotonin both in bacteria and humans, and at phenotypic implications not only manifested in mood behavior but also in infection processes in the host. Thus, regulating serotonin concentrations in the gut may provide with paradigm shifting therapeutic approaches.

Keywords: Host-microbiome interactions; Interkingdom signaling molecule; Las pathway; N-acyl homoserine lactone; Pseudomonas aeruginosa; Quorum sensing; Serotonin.

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism
  • Catalase / metabolism
  • Cytokines / analysis
  • Female
  • Injections, Intraperitoneal
  • Intestinal Mucosa / metabolism
  • Intestines / microbiology
  • Intestines / pathology
  • Malondialdehyde / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron, Scanning
  • Peptide Hydrolases / metabolism
  • Peroxidase / metabolism
  • Phenotype
  • Pregnancy
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / metabolism
  • Pseudomonas aeruginosa / physiology*
  • Quorum Sensing / drug effects*
  • Serotonin / pharmacology*
  • Virulence / drug effects*

Substances

  • Bacterial Proteins
  • Cytokines
  • Serotonin
  • Malondialdehyde
  • Catalase
  • Peroxidase
  • Peptide Hydrolases