Objective: This study aimed to evaluate the vascular endothelial growth factor (VEGF) expression and permeability of vascular endothelial cell under microvibration.
Methods: Human umbilical vein endothelial cell (HUVEC) were cultured, randomly vibrated under low frequency of 0.2, 0.5, 2, 5 Hz, 30 min per day. The VEGF mRNA level was detected by Tagman probe real-time fluorescence quantitative polymerase chain reaction (PCR), and the VEGF protein expression level was detected by Western blot. The permeability of vascular endothelial cell was evaluated.
Results: Compared with the blank control group, the mRNA and protein expression level of VEGF were significantly increased under 0.2, 0.5 Hz thelial, and increase the permeability microvibration (P<0.05), and decreased under 2, 5 Hz microvibration (P<0.01). The vascular endothelial permeability in creased under 0.2, 0.5 Hz microvibration (P<0.01), whereas the permeability decreased under 2, 5 Hz microvibration (P<0.01).
Conclusion: 0.2-0.5 Hz microvibration can up-regulate the expression of VEGF mRNA and protein in vascular endothelial, and increase the permeability.
目的: 研究微振动对血管内皮细胞分泌血管内皮细胞生长因子（VEGF）及通透性的影响。
方法: 培养人脐静脉血管内皮细胞（HUVEC），每日施加0.2、0.5、2、5 Hz 4种低频率微振动30 min，通过Tagman探针实时荧光定量聚合酶链反应（PCR）及蛋白质印迹法检测VEGF的表达，并检测内皮细胞的通透性。
结果: 加载0.2 Hz和0.5 Hz微振动可使血管内皮细胞VEGF mRNA及蛋白表达上调（P<0.05），加载2 Hz和5 Hz振动后VEGF mRNA及蛋白表达下调（P<0.01）；加载0.2 Hz和0.5 Hz微振动可使血管内皮细胞通透性增强（P<0.01），2 Hz和5 Hz微振动则使内皮细胞通透性降低（P<0.01）。
结论: 0.2~0.5 Hz的微振动可使血管内皮细胞分泌VEGF增高，并提高血管内皮细胞的通透性。