Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts

Peggy Matz; James Adjaye

doi:10.1016/j.scr.2015.12.009

Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts

Stem Cell Res. 2016 Jan;16(1):67-9. doi: 10.1016/j.scr.2015.12.009. Epub 2015 Dec 17.

Authors

Peggy Matz¹, James Adjaye²

Affiliations

¹ Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute of Biology, Humboldt University, 10099 Berlin, Germany.
² Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

PMID: 27345787
DOI: 10.1016/j.scr.2015.12.009

Abstract

Human fetal foreskin fibroblasts (HFF1) were used to generate the iPSC line epiHFF1-B1 employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC, and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHFF1-B1 and the human embryonic stem cell line-H1 have a pearson correlation of 0.936.

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Line
DNA Fingerprinting
Fibroblasts / cytology*
Foreskin / cytology*
Human Umbilical Vein Endothelial Cells / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Karyotyping
Kruppel-Like Factor 4
Male
Mice, SCID
Oligonucleotide Array Sequence Analysis
Plasmids / metabolism*