Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Stem Cell Res. 2016 Mar;16(2):300-3. doi: 10.1016/j.scr.2015.12.026. Epub 2016 Jan 4.

Abstract

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.

MeSH terms

  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Comparative Genomic Hybridization
  • DNA Methylation
  • Fibroblasts / cytology*
  • Foreskin / cytology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Kruppel-Like Factor 4
  • Male
  • Microscopy, Fluorescence
  • Octamer Transcription Factor-3 / genetics
  • Promoter Regions, Genetic
  • Real-Time Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • KLF4 protein, human
  • Kruppel-Like Factor 4
  • Octamer Transcription Factor-3
  • POU5F1 protein, human
  • Transcription Factors