Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector

Stem Cell Res. 2016 Mar;16(2):314-7. doi: 10.1016/j.scr.2015.12.018. Epub 2016 Jan 12.

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 30 year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and have shown full differentiation potential. Together with the iPSC-line, the donor provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi004-A.

MeSH terms

  • Adult
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Embryoid Bodies / cytology
  • Epithelial Cells / cytology
  • Female
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Transcription Factors