Intra-operative amylase in peri-pancreatic fluid independently predicts for pancreatic fistula post pancreaticoduodectomy

HPB (Oxford). 2016 Jul;18(7):608-14. doi: 10.1016/j.hpb.2016.05.007. Epub 2016 Jun 16.

Abstract

Background: Post-operative pancreatic fistula (POPF) is a common and potentially life-threatening complication following pancreaticoduodectomy. The aim of this study was to assess the predictive value of intra-operative amylase concentration (IOAC) in peri-pancreatic fluid after resection for the diagnosis of POPF.

Methods: Consecutive patients who underwent a pancreaticoduodectomy between September 2014 and October 2015 were included in the analysis. IOAC was measured intraoperatively followed by drain fluid analysis for amylase on post-operative days (POD) 1, 3 and 5. Receiver operator characteristic (ROC) analysis was performed to evaluate the discriminative capacity of IOAC as a predictor of POPF.

Results: IOAC was measured after pancreaticoduodectomy in 62 patients. The IOAC correlated significantly with i) POD 1 and 3 drain amylase (p < 0.01), ii) the development of POPF (p < 0.01), iii) the development of clinically relevant fistula (Type B, C) (p < 0.01), iv) delayed gastric emptying (p < 0.01), and v) grade of complication as per the Clavien-Dindo definition (p = 0.02). ROC curve analysis confirmed the predictive relationship of IOAC and POPF as a good test with an area under the curve of 0.93, 95% CI 0.87-0.99, p < 0.01. In patients with IOAC of 200 U/L or higher the POPF rate was 80% (OR = 50.1, p < 0.0001).

Discussion: Measurement of IOAC allows early and accurate categorization of patients at risk for POPF.

MeSH terms

  • Aged
  • Amylases / metabolism*
  • Area Under Curve
  • Biomarkers / metabolism
  • Clinical Enzyme Tests*
  • Drainage
  • Female
  • Humans
  • Intraoperative Care
  • Male
  • Middle Aged
  • Pancreatic Fistula / diagnosis
  • Pancreatic Fistula / etiology*
  • Pancreatic Juice / enzymology*
  • Pancreaticoduodenectomy / adverse effects*
  • Predictive Value of Tests
  • ROC Curve
  • Risk Assessment
  • Risk Factors
  • Time Factors
  • Treatment Outcome

Substances

  • Biomarkers
  • Amylases