Self-Sealing Porous Silicon-Calcium Silicate Core-Shell Nanoparticles for Targeted siRNA Delivery to the Injured Brain

Jinyoung Kang; Jinmyoung Joo; Ester J Kwon; Matthew Skalak; Sazid Hussain; Zhi-Gang She; Erkki Ruoslahti; Sangeeta N Bhatia; Michael J Sailor

doi:10.1002/adma.201600634

Self-Sealing Porous Silicon-Calcium Silicate Core-Shell Nanoparticles for Targeted siRNA Delivery to the Injured Brain

Adv Mater. 2016 Sep;28(36):7962-7969. doi: 10.1002/adma.201600634. Epub 2016 Jul 6.

Authors

Jinyoung Kang¹, Jinmyoung Joo², Ester J Kwon³, Matthew Skalak³, Sazid Hussain⁴, Zhi-Gang She⁴, Erkki Ruoslahti^{4

5}, Sangeeta N Bhatia^{3

6

7

8}, Michael J Sailor^{9

10

11}

Affiliations

¹ Department of Nanoengineering, University of California San Diego, La Jolla, CA, 92093-0358, USA.
² Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, 92093-0358, USA.
³ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
⁴ Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA.
⁵ Center for Nanomedicine and Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, 93106-9610, USA.
⁶ Department of Electrical Engineering and Computer Science and David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
⁷ Division of Medicine, Brigham and Women's Hospital, Boston, MA, 02115, USA.
⁸ Howard Hughes Medical Institute, Cambridge, MA, 02139, USA.
⁹ Department of Nanoengineering, University of California San Diego, La Jolla, CA, 92093-0358, USA. [email protected].
¹⁰ Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, 92093-0358, USA. [email protected].
¹¹ Department of Bioengineering, Materials Science and Engineering Program, University of California San Diego, 9500 Gilman Drive, m/c 0358, La Jolla, CA, 92093-0358, USA. [email protected].

Abstract

Calcium ions react with silicic acid released from dissolving porous silicon nanoparticles to create an insoluble calcium silicate shell. The calcium silicate shell traps and protects an siRNA payload, which can be delivered to neuronal tissues in vitro or in vivo. Gene delivery is enhanced by the action of targeting and cell-penetrating peptides attached to the calcium silicate shell.

Keywords: gene knockdowns; injured brains; photoluminescence; rabies virus glycoprotein peptides; targeted nanoparticles.

MeSH terms

Animals
Brain / metabolism
Brain / pathology
Brain Injuries / metabolism*
Brain Injuries / pathology
Calcium Compounds / chemistry*
Cell Line, Tumor
Disease Models, Animal
Mice
Nanoparticles / chemistry*
Porosity
RNA, Small Interfering / administration & dosage*
RNA, Small Interfering / metabolism
RNA, Small Interfering / pharmacokinetics*
Silicates / chemistry*
Silicon / chemistry*

Substances

Calcium Compounds
RNA, Small Interfering
Silicates
calcium silicate
Silicon

Abstract

MeSH terms

Substances

Grants and funding