Plant δ¹⁵ N reflects the high landscape-scale heterogeneity of soil fertility and vegetation productivity in a Mediterranean semiarid ecosystem

We investigated the magnitude and drivers of spatial variability in soil and plant δ¹⁵ N across the landscape in a topographically complex semiarid ecosystem. We hypothesized that large spatial heterogeneity in water availability, soil fertility and vegetation cover would be positively linked to high local-scale variability in δ¹⁵ N. We measured foliar δ¹⁵ N in three dominant plant species representing contrasting plant functional types (tree, shrub, grass) and mycorrhizal association types (ectomycorrhizal or arbuscular mycorrhizal). This allowed us to investigate whether δ¹⁵ N responds to landscape-scale environmental heterogeneity in a consistent way across species. Leaf δ¹⁵ N varied greatly within species across the landscape and was strongly spatially correlated among co-occurring individuals of the three species. Plant δ¹⁵ N correlated tightly with soil δ¹⁵ N and key measures of soil fertility, water availability and vegetation productivity, including soil nitrogen (N), organic carbon (C), plant-available phosphorus (P), water-holding capacity, topographic moisture indices and normalized difference vegetation index. Multiple regression models accounted for 62-83% of within-species variation in δ¹⁵ N across the landscape. The tight spatial coupling and interdependence of the water, N and C cycles in drylands may allow the use of leaf δ¹⁵ N as an integrative measure of variations in moisture availability, biogeochemical activity, soil fertility and vegetation productivity (or 'site quality') across the landscape.