Great genetic diversity of rotaviruses detected in piglets with diarrhea in Thailand

Arch Virol. 2016 Oct;161(10):2843-9. doi: 10.1007/s00705-016-2976-7. Epub 2016 Jul 13.

Abstract

A total of 491 fecal specimens collected from diarrheic piglets in Thailand from January 2011 to March 2014 were screened for group A rotavirus by RT-PCR assay. The G and P genotypes of the detected rotaviruses were determined by multiplex PCR or nucleotide sequencing. Group A rotaviruses were detected in 113 out of 491 (23.0 %) fecal specimens. A wide variety of G-P genotype combinations were identified, and G4P[13] was the most prevalent genotype combination (29.2 %), followed by G4P[23] (14.1 %), G5P[23] (11.5 %), G4P[6] (9.7 %), G3P[23] (7.0 %), G5P[13] (6.1 %), G3P[13] (4.4 %), G3P[6] (2.7 %), and G5P[6] (2.7 %). In addition, the other G-P combinations were also detected at a low percentage, including G3P[19], G4P[7], G9P[19], G9P[23], G9P[7], G4P[19], and G11P[13] strains. This study indicated that group A rotaviruses are a common causes of diarrhea in piglets and a great diversity of G and P genotype combinations are circulating in piglets in Thailand.

Keywords: Diarrhea; Piglet; Rotavirus; Thailand.

MeSH terms

  • Animals
  • Animals, Newborn
  • Diarrhea / epidemiology
  • Diarrhea / veterinary*
  • Diarrhea / virology
  • Feces / virology
  • Genetic Variation*
  • Genotype*
  • Multiplex Polymerase Chain Reaction
  • Prevalence
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / veterinary*
  • Rotavirus Infections / virology
  • Swine
  • Swine Diseases / epidemiology
  • Swine Diseases / virology*
  • Thailand / epidemiology