Amelioration of EAE by a cryptic epitope of myelin oligodendrocyte glycoprotein

J Neuroimmunol. 2016 Nov 15:300:66-73. doi: 10.1016/j.jneuroim.2016.06.006. Epub 2016 Jun 24.

Abstract

Previous work demonstrated that EAE induced by recombinant human MOG was B cell-dependent. Data presented here reveal a T cell response to MOG61-85 in human rMOG-immunized B cell-/- mice not observed in WT mice. Further study revealed this peptide to be a cryptic epitope in WT mice. Co-immunization of B cell-/- mice with MOG35-55 and MOG61-85 peptides led to less severe disease compared to mice immunized with MOG35-55 alone. Disease amelioration was associated with decreased production of Interferon-γ by lymph node cells. Thus, MOG61-85 represents a protective epitope to human rMOG induced EAE in B cell-/- mice.

Keywords: Antibody; B cells; Experimental autoimmune encephalomyelitis; Immune regulation; Myelin oligodendrocyte glycoprotein; T cell epitopes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Encephalomyelitis, Autoimmune, Experimental / drug therapy*
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Epitopes / administration & dosage*
  • Epitopes / immunology*
  • Female
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myelin-Oligodendrocyte Glycoprotein / administration & dosage*
  • Myelin-Oligodendrocyte Glycoprotein / immunology*

Substances

  • Epitopes
  • Myelin-Oligodendrocyte Glycoprotein