First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Laurent Dortet; Rémy A Bonnin; Sandrine Bernabeu; Lélia Escaut; Daniel Vittecoq; Delphine Girlich; Dilek Imanci; Nicolas Fortineau; Thierry Naas

doi:10.1128/AAC.01016-16

First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Antimicrob Agents Chemother. 2016 Sep 23;60(10):5724-30. doi: 10.1128/AAC.01016-16. Print 2016 Oct.

Authors

Laurent Dortet¹, Rémy A Bonnin², Sandrine Bernabeu³, Lélia Escaut⁴, Daniel Vittecoq⁴, Delphine Girlich⁵, Dilek Imanci⁶, Nicolas Fortineau³, Thierry Naas³

Affiliations

¹ Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France [email protected].
² French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
³ Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
⁴ Infectious Diseases Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
⁵ EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
⁶ Molecular Genetics and Hormonology Department, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.

Abstract

Here, we characterized the first OXA-72-producing Acinetobacter baumannii isolate (designated MAL) recovered from a urine sample from a Serbian patient. Antimicrobial susceptibility testing, plasmid analysis, and whole-genome sequencing (WGS) were performed to fully characterize the resistome of the A. baumannii MAL clinical isolate. The isolate was multidrug resistant and remained susceptible only to colistin and tigecycline. PCR analysis revealed the presence of the carbapenemase OXA-72, an OXA-40 variant. Extraction by the Kieser method revealed the presence of two plasmids, and one of these, a ca. 10-kb plasmid, harbored the blaOXA-72 gene. WGS revealed 206 contigs corresponding to a genome of 3.9 Mbp in size with a G+C content of 38.8%. The isolate belonged to sequence type 492 and to worldwide clone II (WWCII). Naturally occurring β-lactamase-encoding genes (blaADC-25 and blaOXA-66) were also identified. Aminoglycoside resistance genes encoding one aminoglycoside adenyltransferase (aadA2), three aminoglycoside phosphatases (strA, strB, aphA6), and one 16S RNA methylase (armA) conferring resistance to all aminoglycosides were identified. Resistance to fluoroquinolones was likely due to mutations in gyrA, parC, and parE Of note, the resistome matched perfectly with the antibiotic susceptibility testing results.

MeSH terms

Acinetobacter Infections / drug therapy
Acinetobacter Infections / microbiology*
Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / genetics
Acinetobacter baumannii / isolation & purification*
Acinetobacter baumannii / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Drug Resistance, Multiple, Bacterial / drug effects
Drug Resistance, Multiple, Bacterial / genetics
Humans
Microbial Sensitivity Tests
Plasmids
Serbia
Urinary Tract Infections / drug therapy
Urinary Tract Infections / microbiology
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
OXA-72 carbapenemase, Acinetobacter baumannii
beta-Lactamases

Grants and funding

This work was partially funded by grants from the University Paris-Sud, France, from the European Community (MAGIC-BULLET, FP7/HEALTH-F3-2001-27823), and from the Joint Program Initiative on Antimicrobial Resistance (ANR-14-JAMR-0002). L.D., S.B., D.G., N.F., and T.N. are members of the Laboratory of Excellence LERMIT, supported by a grant from ANR (ANR-10-LABX-33).