Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran: A 5-year survey

Sharereh Khanipour; Nayereh Ebrahimzadeh; Morteza Masoumi; Fatemeh Sakhaei; Farhad Alinezhad; Elham Safarpour; Abolfazl Fateh; Ali Nour Nematollahi; Alireza Hadizadeh Tasbiti; Mohammad Reza Zolfaghari; Ahmad Reza Bahrmand; Mehdi Mirsaeidi; Fatemeh Rahimi Jamnani; Farzam Vaziri; Seyed Davar Siadat

doi:10.1016/j.jgar.2016.01.007

Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran: A 5-year survey

J Glob Antimicrob Resist. 2016 Jun:5:7-10. doi: 10.1016/j.jgar.2016.01.007. Epub 2016 Feb 26.

Authors

Sharereh Khanipour¹, Nayereh Ebrahimzadeh², Morteza Masoumi¹, Fatemeh Sakhaei², Farhad Alinezhad², Elham Safarpour², Abolfazl Fateh², Ali Nour Nematollahi², Alireza Hadizadeh Tasbiti², Mohammad Reza Zolfaghari³, Ahmad Reza Bahrmand², Mehdi Mirsaeidi⁴, Fatemeh Rahimi Jamnani⁵, Farzam Vaziri⁶, Seyed Davar Siadat⁵

Affiliations

¹ Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran; Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
² Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran.
³ Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
⁴ Division of Pulmonary and Critical Care, University of Miami, Miami, FL, USA.
⁵ Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
⁶ Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran. Electronic address: [email protected].

PMID: 27436458
DOI: 10.1016/j.jgar.2016.01.007

Abstract

The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. The results showed that the MDR, pre-XDR and XDR M. tuberculosis strains mainly belonged to the Haarlem 3 genotype (11/23; 47.8%), followed by the Beijing family (9/23; 39.1%). In addition, the 23 strains were clustered into 21 genotypes using a 24-loci MIRU-VNTR. In conclusion, Haarlem 3 genotype was the predominant genotype among the isolates from MDR-TB cases in this study, which could be of special concern.

Keywords: 24-Loci MIRU-VNTR; Haarlem 3; MDR-TB; Spoligotyping.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Extensively Drug-Resistant Tuberculosis / microbiology*
Female
Genotype*
Humans
Iran
Male
Middle Aged
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Tuberculosis, Multidrug-Resistant / microbiology*
Young Adult