TMEM231 Gene Conversion Associated with Joubert and Meckel-Gruber Syndromes in the Same Family

Hum Mutat. 2016 Nov;37(11):1144-1148. doi: 10.1002/humu.23054. Epub 2016 Aug 21.

Abstract

Joubert and Meckel-Gruber syndromes (JS and MGS) are ciliopathies with overlapping features. JS patients manifest the "molar tooth sign" on brain imaging and variable eye, kidney, and liver disease. MGS presents with polycystic kidneys, occipital encephalocele, and polydactyly; it is typically perinatally fatal. Both syndromes are genetically heterogeneous; some genes cause either syndrome. Here, we report two brothers married to unrelated women. The first brother had three daughters with JS and a son with polycystic kidneys who died at birth. The second brother's wife had a fetal demise due to MGS. Whole exome sequencing identified TMEM231 NM_001077416.2: c.784G>A; p.(Asp262Asn) in all children and the wife of the first brother; the second brother's wife had a c.406T>G;p.(Trp136Gly) change. In-depth analysis uncovered a rare gene conversion event in TMEM231, leading to loss of exon 4, in all the affected children of first brother. We believe that the combination of this gene conversion with different missense mutations led to a spectrum of phenotypes that span JS and MGS.

Keywords: TMEM231; cilia; gene conversion; translocation; whole exome sequencing.

MeSH terms

  • Abnormalities, Multiple / genetics*
  • Cerebellum / abnormalities*
  • Ciliary Motility Disorders / genetics*
  • Encephalocele / genetics*
  • Exome
  • Eye Abnormalities / genetics*
  • Female
  • Gene Conversion*
  • Humans
  • Kidney Diseases, Cystic / genetics*
  • Male
  • Membrane Proteins / genetics*
  • Mutation, Missense
  • Pedigree
  • Polycystic Kidney Diseases / genetics*
  • Retina / abnormalities*
  • Retinitis Pigmentosa
  • Sequence Analysis, DNA / methods

Substances

  • Membrane Proteins
  • TMEM231 protein, human

Supplementary concepts

  • Agenesis of Cerebellar Vermis
  • Meckel syndrome type 1