Proposal of Mucilaginibacter phyllosphaerae sp. nov. isolated from the phyllosphere of Galium album

Int J Syst Evol Microbiol. 2016 Oct;66(10):4138-4147. doi: 10.1099/ijsem.0.001326. Epub 2016 Jul 27.

Abstract

A pink-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterial strain, PP-F2F-G21T, was isolated from the phyllosphere of Galium album. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence of strain PP-F2F-G21T showed the closest relationship to type strains of the species Mucilaginibacter lutimaris (97.7 %), Mucilaginibacter soli (97.3 %) and Mucilaginibacter rigui (97.1 %). Sequence similarities to all other type strains were below 97 %. The predominant cellular fatty acids of strain PP-F2F-G21T are C16 : 1 ω7c/iso-C15 : 0 2-OH (measured as summed feature 3 fatty acids) and iso-C15 : 0 followed by iso-C17 : 0 3-OH, C16 : 1 ω5c and C16 : 0. The major compound in the polyamine pattern was sym-homospermidine and the diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system was exclusively composed of menaquinone MK-7. The polar lipid profile contained the major lipid phosphatidylethanolamine and in addition 18 unidentified lipids. Based on phylogenetic, chemotaxonomic and phenotypic analyses, we propose a novel species of the genus Mucilaginibacter named Mucilaginibacter phyllosphaeraesp. nov. The type strain is PP-F2F-G21T (=CCM 8625T=CIP 110921T=LMG 29118T).

MeSH terms

  • Bacterial Typing Techniques
  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Galium / microbiology*
  • Germany
  • Phosphatidylethanolamines / chemistry
  • Phylogeny*
  • Pigmentation
  • Plant Leaves / microbiology
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Spermidine / analogs & derivatives
  • Spermidine / chemistry
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • RNA, Ribosomal, 16S
  • sym-homospermidine
  • Vitamin K 2
  • phosphatidylethanolamine
  • Diaminopimelic Acid
  • menaquinone 7
  • Spermidine