Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source

Sci Data. 2016 Aug 1:3:160064. doi: 10.1038/sdata.2016.64.

Abstract

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

Publication types

  • Dataset
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Oryza / virology*
  • Particle Accelerators
  • Reoviridae / isolation & purification*
  • Virion*
  • X-Rays

Associated data

  • figshare/10.6084/M9.FIGSHARE.C.2342581