Does molecular analysis increase the efficacy of bronchoalveolar lavage in the diagnosis and management of respiratory infections in hemato-oncological patients?

Int J Infect Dis. 2016 Sep:50:48-53. doi: 10.1016/j.ijid.2016.07.011. Epub 2016 Jul 30.

Abstract

Objectives: The identification of the specific pathogen responsible for a respiratory infection in patients with hematological malignancies (HM) would ensure relevant treatment and prevent toxicity associated with anti-infective therapy. This large-scale study aimed to explore the clinical impact of fiberoptic bronchoscopy with bronchoalveolar lavage (FOB-BAL) in conjunction with molecular analysis on the diagnosis and management of respiratory infections in hemato-oncological patients.

Methods: All consecutive patients with HM and pulmonary infiltrates, who underwent FOB-BAL between January 2008 and January 2013, were included in the analysis. Clinical characteristics, FOB-BAL results, and treatment adjustments were recorded, and factors predicting a positive BAL were assessed.

Results: Four hundred and twenty-five FOB-BAL procedures were analyzed. BAL revealed a specific diagnosis in 219 (51.5%) patients, 208 of them with a pulmonary infection. Infectious etiological agents found were mainly Aspergillus spp (n=142), bacterial species (n=44), and Pneumocystis jirovecii (n=34). Multivariate analysis showed that a lymphoproliferative disease, ≥2 symptoms (dyspnea/cough/hemoptysis/pleuritic pain), and less than 4 days between symptom appearance and FOB-BAL, predicted a positive FOB-BAL result. BAL results prompted a treatment modification in 48% of subjects.

Conclusions: FOB-BAL in conjunction with molecular assays is efficient in the rapid detection of life-threatening infections, allowing for adjustment of anti-infective therapy, which may result in better outcomes and reduce treatment-related toxicity.

Keywords: Fiberoptic bronchoscopy with bronchoalveolar lavage; Hematological malignancies; Molecular analysis; Respiratory infections.

Publication types

  • Clinical Trial

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Bronchoalveolar Lavage / methods*
  • Bronchoalveolar Lavage Fluid / microbiology*
  • Bronchoscopy / methods*
  • Female
  • Fungi / classification
  • Fungi / genetics
  • Fungi / isolation & purification
  • Hematologic Neoplasms / complications*
  • Humans
  • Male
  • Middle Aged
  • Respiratory Tract Infections / diagnosis*
  • Respiratory Tract Infections / etiology
  • Respiratory Tract Infections / microbiology
  • Young Adult