A recurring mutation in the respiratory complex 1 protein NDUFB11 is responsible for a novel form of X-linked sideroblastic anemia

Blood. 2016 Oct 13;128(15):1913-1917. doi: 10.1182/blood-2016-05-719062. Epub 2016 Aug 3.

Abstract

The congenital sideroblastic anemias (CSAs) are a heterogeneous group of inherited blood disorders characterized by pathological mitochondrial iron deposition in erythroid precursors. Each known cause has been attributed to a mutation in a protein associated with heme biosynthesis, iron-sulfur cluster biogenesis, mitochondrial translation, or a component of the mitochondrial respiratory chain. Here, we describe a recurring mutation, c.276_278del, p.F93del, in NDUFB11, a mitochondrial respiratory complex I-associated protein encoded on the X chromosome, in 5 males with a variably syndromic, normocytic CSA. The p.F93del mutation results in respiratory insufficiency and loss of complex I stability and activity in patient-derived fibroblasts. Targeted introduction of this allele into K562 erythroleukemia cells results in a proliferation defect with minimal effect on erythroid differentiation potential, suggesting the mechanism of anemia in this disorder.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Anemia, Sideroblastic / genetics*
  • Anemia, Sideroblastic / metabolism
  • Anemia, Sideroblastic / pathology
  • Base Sequence*
  • Child
  • Child, Preschool
  • Chromosomes, Human, X / genetics*
  • Chromosomes, Human, X / metabolism
  • Electron Transport Complex I / genetics*
  • Electron Transport Complex I / metabolism
  • Female
  • Genetic Diseases, X-Linked / genetics*
  • Genetic Diseases, X-Linked / metabolism
  • Humans
  • K562 Cells
  • Male
  • Middle Aged
  • Sequence Deletion*

Substances

  • NDUFB11 protein, human
  • Electron Transport Complex I