Isolation of Treponema DNA from Necrophagous Flies in a Natural Ecosystem

EBioMedicine. 2016 Sep:11:85-90. doi: 10.1016/j.ebiom.2016.07.033. Epub 2016 Jul 28.

Abstract

Background: Recently, the World Health Organization launched a campaign to eradicate the tropical disease yaws, caused by the bacterium Treponema pallidum subsp. pertenue; however, for decades researchers have questioned whether flies act as a vector for the pathogen that could facilitate transmission.

Methods: A total of 207 fly specimens were trapped in areas of Africa in which T. pallidum-induced skin ulcerations are common in wild baboons; 88 flies from Tarangire National Park and 119 from Lake Manyara National Park in Tanzania were analyzed by PCR for the presence of T. pallidum DNA.

Findings: We report that in the two study areas, T. pallidum DNA was found in 17-24% of wild-caught flies of the order Diptera. Treponemal DNA sequences obtained from many of the flies match sequences derived from nearby baboon T. pallidum strains, and one of the fly species with an especially high prevalence of T. pallidum DNA, Musca sorbens, has previously been shown to transmit yaws in an experimental setting.

Interpretation: Our results raise the possibility that flies play a role in yaws transmission; further research is warranted, given how important understanding transmission is for the eradication of this disfiguring disease.

Keywords: Dipteria; Nonhuman primates; Transmission; Treponema pallidum; Yaws.

MeSH terms

  • Africa
  • Animals
  • DNA, Bacterial*
  • Diptera / classification
  • Diptera / microbiology*
  • Ecosystem*
  • Environmental Microbiology
  • Genes, Bacterial
  • Insect Vectors / microbiology
  • Papio / microbiology
  • Phylogeny
  • Sequence Analysis, DNA
  • Tanzania
  • Treponema / genetics*

Substances

  • DNA, Bacterial