Chloroplast biogenesis: quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry

S M Wu; J M Mayasich; C A Rebeiz

doi:10.1016/0003-2697(89)90641-6

Chloroplast biogenesis: quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry

Anal Biochem. 1989 May 1;178(2):294-300. doi: 10.1016/0003-2697(89)90641-6.

Authors

S M Wu¹, J M Mayasich, C A Rebeiz

Affiliation

¹ Laboratory of Plant Pigment Biochemistry and Photobiology, University of Illinois, Urbana 61801.

PMID: 2751091
DOI: 10.1016/0003-2697(89)90641-6

Abstract

Simultaneous equations for the determination of monovinyl (MV) and divinyl (DV) Chl a and b by spectrofluorometry in unsegregated mixtures of these tetrapyrroles were derived. The same equations can also be used for the quantitative determination of MV and DV chlorophyllide (Chlide) a and b in unpurified mixtures, after extraction of the Chls in hexane. The equations used differences in the Soret excitation maxima of these tetrapyrroles in ether at 77 degrees K, in order to correct the Soret excitation overlap between MV and DV Chl(ide) a and between MV and DV Chl(ide) b.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chlorophyll / analogs & derivatives*
Chlorophyllides / analysis*
Chlorophyllides / biosynthesis
Chloroplasts / metabolism*
Mathematics
Spectrometry, Fluorescence

Substances

Chlorophyllides
Chlorophyll