Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures

Adem Ozcelik; Nitesh Nama; Po-Hsun Huang; Murat Kaynak; Melanie R McReynolds; Wendy Hanna-Rose; Tony Jun Huang

doi:10.1002/smll.201601760

Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures

Small. 2016 Oct;12(37):5120-5125. doi: 10.1002/smll.201601760. Epub 2016 Aug 12.

Authors

Adem Ozcelik¹, Nitesh Nama², Po-Hsun Huang¹, Murat Kaynak², Melanie R McReynolds³, Wendy Hanna-Rose³, Tony Jun Huang⁴

Affiliations

¹ Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
² Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.
³ Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA.
⁴ Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA. [email protected].

Abstract

A polydimethylsiloxane microchannel featuring sidewall sharp-edge structures and bare channels, and a piezoelement transducer is attached to a thin glass slide. When an external acoustic field is applied to the microchannel, the oscillation of the sharp-edge structures and the thin glass slide generate acoustic streaming flows which in turn rotate single cells and C. elegans in-plane and out-of-plane.

Keywords: C. elegans; acoustofluidics; microfluidics; rotational manipulations.

MeSH terms

Acoustics / instrumentation*
Animals
Caenorhabditis elegans / cytology*
HeLa Cells
Humans
Neurons / cytology
Rotation*

Abstract

MeSH terms

Grants and funding