Sulfated polysaccharide fraction from marine algae Solieria filiformis: Structural characterization, gastroprotective and antioxidant effects

Willer M Sousa; Renan O Silva; Francisco F Bezerra; Rudy D Bingana; Francisco Clark N Barros; Luís E C Costa; Venicios G Sombra; Pedro M G Soares; Judith P A Feitosa; Regina C M de Paula; Marcellus H L P Souza; André Luiz R Barbosa; Ana Lúcia P Freitas

doi:10.1016/j.carbpol.2016.06.111

Sulfated polysaccharide fraction from marine algae Solieria filiformis: Structural characterization, gastroprotective and antioxidant effects

Carbohydr Polym. 2016 Nov 5:152:140-148. doi: 10.1016/j.carbpol.2016.06.111. Epub 2016 Jun 29.

Affiliations

¹ Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil.
² LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
³ Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil; Federal Institute of Education, Science and Technology of Ceará, Juazeiro do Norte, CE, Brazil.
⁴ Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil.
⁵ LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil; Department of Morphology, Medical School, Federal University of Ceara, Rua Delmiro de Farias s/n, Rodolfo Teofilo, Fortaleza, CE CEP 60416-030, Brazil.
⁶ LAFFEX - Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020, PI, Brazil. Electronic address: [email protected].

PMID: 27516258
DOI: 10.1016/j.carbpol.2016.06.111

Abstract

A sulfated polysaccharide (SFP) fraction from the marine alga Solieria filiformis was extracted and submitted to microanalysis, molar mass estimation and spectroscopic analysis. We evaluated its gastroprotective potential in vivo in an ethanol-induced gastric damage model and its in vitro antioxidant properties (DPPH, chelating ferrous ability and total antioxidant capacity). Its chemical composition revealed to be essentially an iota-carrageenan with a molar mass of 210.9kDa and high degree of substitution for sulfate groups (1.08). In vivo, SFP significantly (P<0.05) reduced, in a dose dependent manner, the ethanol-induced gastric damage. SFP prevents glutathione consume and increase of malondialdehyde and hemoglobin levels. SFP presented an IC50 of 1.77mg/mL in scavenging DPPH. The chelating ferrous ability was 38.98%, and the total antioxidant capacity was 2.01mg/mL. Thus, SFP prevents the development of ethanol-induced gastric damage by reducing oxidative stress in vivo and possesses relevant antioxidant activity in vitro.

Keywords: Antioxidant; Ethanol; Polysaccharides; Solieria filiformis.

MeSH terms

Animals
Antioxidants* / chemistry
Antioxidants* / isolation & purification
Antioxidants* / pharmacology
Ethanol / toxicity
Mice
Oxidative Stress / drug effects*
Polysaccharides* / chemistry
Polysaccharides* / isolation & purification
Polysaccharides* / pharmacology
Rhodophyta / chemistry*
Stomach Diseases / chemically induced
Stomach Diseases / prevention & control*

Substances

Antioxidants
Polysaccharides
Ethanol