Changes in biomarkers during a six-month oral immunotherapy intervention for cow's milk allergy

Acta Paediatr. 2016 Nov;105(11):1349-1354. doi: 10.1111/apa.13550.

Abstract

Aim: Oral immunotherapy (OIT) is a promising but still experimental method to treat children with cow's milk (CM) allergy (CMA). We evaluated changes in allergic, immunological and inflammatory parameters, which happened during the six-month OIT for CMA.

Methods: We treated 28 school-aged children with CMA using OIT with a double-blind placebo-controlled design. After the controlled study finished, the placebo group was treated with the same but open-label OIT protocol. Sixteen immune variables were tested before and after the six-month OIT.

Results: Before OIT, the median serum CM-specific immunoglobulin (Ig) E was 18.0kIU/L in the intervention group and 9.4kIU/L in the placebo group (p = 0.46). At six months, interleukin (IL)-6 and IL-10 were significantly higher in the intervention group. When the changes during the blinded and open OIT were analysed together for both groups, blood eosinophils and serum total IgE decreased and milk-specific IgG and IgG4, serum IL-4 and IL-6, and serum leptin and resistin increased significantly.

Conclusion: Preliminary evidence was found that markers of allergy such as blood eosinophils and serum IgE decreased and milk-specific IgG and IgG4 increased during OIT. Adipokines, leptin and resistin, which functionally are cytokines linked to Th1-type response, increased during OIT.

Keywords: Adipokines; Cow's milk allergy; Cytokines; Eosinophils; Immunoglobulin E; Oral immunotherapy.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Adolescent
  • Animals
  • Biomarkers / blood*
  • Cattle
  • Child
  • Cytokines / blood
  • Cytokines / immunology*
  • Desensitization, Immunologic / adverse effects
  • Desensitization, Immunologic / methods*
  • Eosinophils / immunology
  • Humans
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunotherapy / methods*
  • Interleukin-10 / blood
  • Interleukin-10 / immunology
  • Interleukin-6 / blood
  • Interleukin-6 / immunology
  • Milk / adverse effects
  • Milk / immunology
  • Milk Hypersensitivity / blood
  • Milk Hypersensitivity / immunology
  • Milk Hypersensitivity / therapy*
  • Th1 Cells / immunology
  • Th2 Cells / immunology

Substances

  • Biomarkers
  • Cytokines
  • Immunoglobulin G
  • Interleukin-6
  • Interleukin-10
  • Immunoglobulin E