Clinical features and molecular epidemiology of plasmid-mediated DHA-type AmpC β-lactamase-producing Klebsiella pneumoniae blood culture isolates, Hong Kong

J Glob Antimicrob Resist. 2016 Dec:7:37-42. doi: 10.1016/j.jgar.2016.06.006. Epub 2016 Jul 28.

Abstract

Knowledge of risk factors and clinical characteristics of bacteraemia caused by plasmid-mediated AmpC β-lactamase (pAmpC)-producing Klebsiella pneumoniae (pAmpC-Kp) is not well described. This was a retrospective cohort study of patients with K. pneumoniae bacteraemia in three Hong Kong regional hospitals. Demographic and clinical data were retrieved from medical records. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed for molecular epidemiology. A total of 109 patients were included, divided into four groups: bacteraemia due to K. pneumoniae with (i) DHA-type pAmpC (n=23), (ii) extended-spectrum β-lactamase (ESBL) (n=37), (iii) DHA-type pAmpC+ESBL (n=26) and (iv) controls (n=23). Nursing home residence was independently associated with pAmpC-Kp bacteraemia compared with ESBL-Kp bacteraemia [adjusted odds ratio (aOR)=7.13, 95% confidence interval (CI) 1.36-37.54] and controls (aOR=41.47, 95% CI 4.55-377.75). Compared with controls, patients with pAmpC-Kp bacteraemia also suffered from more severe illness [median Acute Physiology and Chronic Health Evaluation (APACHE) II scores 16 and 25, respectively; P=0.006]. Importantly, the pAmpC group received discordant empirical antimicrobial therapy more frequently (OR=24.00, 95% CI 5.01-114.97), resulting in higher 7-day mortality (OR=20.17, 95% CI 2.32-175.67) and 30-day mortality (OR 4.68, 95% CI 1.29-16.98). PFGE detected six pulsotypes, corresponding to the predominant sequence type 11. Severity of illness and mortality of patients with bacteraemia caused by pAmpC-Kp were high. Patients who are nursing home residents presenting nosocomial sepsis should be treated with broad-spectrum antimicrobials.

Keywords: Bacteraemia; Clinical features; Hong Kong; Klebsiella pneumoniae; Molecular epidemiology; Plasmid-mediated AmpC β-lactamase.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Bacterial Proteins / genetics*
  • Blood Culture
  • Female
  • Hong Kong
  • Humans
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Male
  • Middle Aged
  • Molecular Epidemiology*
  • Multilocus Sequence Typing
  • Nursing Homes
  • Plasmids / genetics
  • Retrospective Studies
  • Risk Factors
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • AmpC beta-lactamases
  • beta-Lactamases