A Multicomponent Animal Virus Isolated from Mosquitoes

Jason T Ladner; Michael R Wiley; Brett Beitzel; Albert J Auguste; Alan P Dupuis 2nd; Michael E Lindquist; Samuel D Sibley; Krishna P Kota; David Fetterer; Gillian Eastwood; David Kimmel; Karla Prieto; Hilda Guzman; Matthew T Aliota; Daniel Reyes; Ernst E Brueggemann; Lena St John; David Hyeroba; Michael Lauck; Thomas C Friedrich; David H O'Connor; Marie C Gestole; Lisa H Cazares; Vsevolod L Popov; Fanny Castro-Llanos; Tadeusz J Kochel; Tara Kenny; Bailey White; Michael D Ward; Jose R Loaiza; Tony L Goldberg; Scott C Weaver; Laura D Kramer; Robert B Tesh; Gustavo Palacios

doi:10.1016/j.chom.2016.07.011

A Multicomponent Animal Virus Isolated from Mosquitoes

Cell Host Microbe. 2016 Sep 14;20(3):357-367. doi: 10.1016/j.chom.2016.07.011. Epub 2016 Aug 25.

Authors

Jason T Ladner¹, Michael R Wiley², Brett Beitzel², Albert J Auguste³, Alan P Dupuis 2nd⁴, Michael E Lindquist⁵, Samuel D Sibley⁶, Krishna P Kota⁷, David Fetterer⁸, Gillian Eastwood⁴, David Kimmel², Karla Prieto², Hilda Guzman³, Matthew T Aliota⁴, Daniel Reyes², Ernst E Brueggemann⁷, Lena St John², David Hyeroba⁹, Michael Lauck¹⁰, Thomas C Friedrich¹¹, David H O'Connor¹⁰, Marie C Gestole², Lisa H Cazares¹², Vsevolod L Popov³, Fanny Castro-Llanos¹³, Tadeusz J Kochel¹³, Tara Kenny⁷, Bailey White², Michael D Ward⁷, Jose R Loaiza¹⁴, Tony L Goldberg¹⁵, Scott C Weaver³, Laura D Kramer¹⁶, Robert B Tesh³, Gustavo Palacios¹⁷

Affiliations

¹ Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. Electronic address: [email protected].
² Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
³ Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
⁴ Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA.
⁵ Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
⁶ Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
⁷ Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
⁸ Research Support Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
⁹ Makerere University, Kampala, Uganda.
¹⁰ Wisconsin National Primate Research Center, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
¹¹ Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA; Wisconsin National Primate Research Center, Madison, WI 53715, USA.
¹² Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA; Henry M. Jackson Foundation, Bethesda, MD 20817, USA; DoD Biotechnology High Performance Computing Software Applications Institute, Frederick, MD 21702, USA; Telemedicine and Advanced Technology Research Center, U.S. Army Medical Research and Materiel Command, Fort Detrick, MD 21702, USA.
¹³ U.S. Naval Medical Research Unit No. 6, Lima, Peru.
¹⁴ Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Ciudad de Panamá, Panamá.
¹⁵ Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA; Makerere University, Kampala, Uganda; Wisconsin National Primate Research Center, Madison, WI 53715, USA.
¹⁶ Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA; School of Public Health, State University of New York at Albany, One University Place Rensselaer, East Greenbush, NY 12144, USA.
¹⁷ Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. Electronic address: [email protected].

Abstract

RNA viruses exhibit a variety of genome organization strategies, including multicomponent genomes in which each segment is packaged separately. Although multicomponent genomes are common among viruses infecting plants and fungi, their prevalence among those infecting animals remains unclear. We characterize a multicomponent RNA virus isolated from mosquitoes, designated Guaico Culex virus (GCXV). GCXV belongs to a diverse clade of segmented viruses (Jingmenvirus) related to the prototypically unsegmented Flaviviridae. The GCXV genome comprises five segments, each of which appears to be separately packaged. The smallest segment is not required for replication, and its presence is variable in natural infections. We also describe a variant of Jingmen tick virus, another Jingmenvirus, sequenced from a Ugandan red colobus monkey, thus expanding the host range of this segmented and likely multicomponent virus group. Collectively, this study provides evidence for the existence of multicomponent animal viruses and their potential relevance for animal and human health.

MeSH terms

Animals
Colobus / virology*
Culicidae / virology*
Microscopy, Fluorescence
Phylogeny
RNA Viruses / classification
RNA Viruses / genetics
RNA Viruses / isolation & purification*
RNA Viruses / ultrastructure*
Viruses / classification
Viruses / genetics
Viruses / isolation & purification*
Viruses / ultrastructure*

Abstract

MeSH terms

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