Comparison of lung damage in mice exposed to black carbon particles and ozone-oxidized black carbon particles

Hongqian Chu; Jing Shang; Ming Jin; Qian Li; Yueyue Chen; Hongpeng Huang; Yuan Li; Yao Pan; Xi Tao; Zhiyuan Cheng; Qinghe Meng; Guang Jia; Tong Zhu; Xuetao Wei; Weidong Hao

doi:10.1016/j.scitotenv.2016.08.137

Comparison of lung damage in mice exposed to black carbon particles and ozone-oxidized black carbon particles

Sci Total Environ. 2016 Dec 15:573:303-312. doi: 10.1016/j.scitotenv.2016.08.137. Epub 2016 Aug 26.

Authors

Hongqian Chu¹, Jing Shang², Ming Jin¹, Qian Li³, Yueyue Chen², Hongpeng Huang¹, Yuan Li¹, Yao Pan¹, Xi Tao¹, Zhiyuan Cheng¹, Qinghe Meng¹, Guang Jia⁴, Tong Zhu², Xuetao Wei⁵, Weidong Hao⁶

Affiliations

¹ Department of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR China.
² State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China.
³ State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China; POTEN Environment Group Co., Ltd., Beijing 100082, PR China.
⁴ Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing 100191, PR China.
⁵ Department of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR China. Electronic address: [email protected].
⁶ Department of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR China. Electronic address: [email protected].

PMID: 27570198
DOI: 10.1016/j.scitotenv.2016.08.137

Abstract

Black carbon (BC) is a key component of atmospheric particles and has a significant effect on human health. Oxidation could change the characteristics of BC and increase its toxicity. The comparison of lung damage in mice exposed to BC and ozone-oxidized BC (oBC) particles is investigated in this study. Mice which were intratracheally instilled with particles have a higher expression of IL-1β, IL-6 and IL-33 in bronchoalveolar lavage fluid (BALF). Also, the IL-6, IL-33 mRNA expression in the lung tissue of mice instilled with oBC was higher than that of mice instilled with BC. The expression of CD3 in the lung tissue of mice intratracheally instilled with oBC was higher than the mice distilled with BC. The pathology results showed that the lung tissue of mice instilled with oBC particles have much more inflammatory cells infiltration than that of mice treated with BC. It is believed that the PI3K-AKT pathway might be involved in the oBC particles caused lung damage. Results indicated that oBC particles in the atmosphere may cause more damage to health.

Keywords: Black carbon; Il-33; Lung; oBC.

MeSH terms

Animals
Bronchoalveolar Lavage Fluid / chemistry
Bronchoalveolar Lavage Fluid / immunology
Female
Inhalation Exposure / adverse effects*
Lung / drug effects*
Lung / immunology
Lung / pathology
Mice, Inbred C57BL
Oxidation-Reduction
Ozone / chemistry*
Particulate Matter / chemistry
Particulate Matter / toxicity*
Soot / chemistry
Soot / toxicity*

Substances

Particulate Matter
Soot
Ozone