Intensity-specific effect of physical activity on urinary levels of 8-hydroxydeoxyguanosine in middle-aged Japanese

Cancer Sci. 2016 Nov;107(11):1653-1659. doi: 10.1111/cas.13070.

Abstract

Physical activity (PA) is recommended to both promote and maintain health and prevent cancer by improving the body's DNA repair system, which is considered a mechanism of cancer prevention. However, associations between PA and urinary levels of 8-hydroxydeoxyguanosine (8-OH-dG), which reflects DNA damage, are unclear. This cross-sectional study included 2370 men and 4052 women aged 45-74 years enrolled between 2010 and 2012. Habitual PA was assessed by single-axis accelerometer and urinary 8-OH-dG levels by automated HPLC. Multiple linear regression analysis was used to examine the relationship between log-transformed urinary 8-OH-dG and total PA (TPA) and PA of moderate/vigorous intensity (MVPA; ≥3 metabolic equivalents), with adjustment for age, body mass index, energy intake, alcohol consumption, smoking status, daily coffee drinking, menopause status (in women), and TPA (for MVPA). On multivariate adjustment, urinary 8-OH-dG levels were inversely correlated with TPA (β = -0.020, P < 0.01) in women, and this correlation was not changed by PA intensity. Conversely, urinary 8-OH-dG levels were inversely correlated with MVPA (β = -0.022, P < 0.05) in men, although the correlation with TPA was non-significant. This inverse correlation was clearer in current smokers than in never or former smokers, although the interaction between smoking status and MVPA on urinary 8-OH-dG levels was non-significant. In conclusion, greater TPA in women and greater MVPA in men were correlated with reduction in urinary 8-OH-dG, suggesting sex-specific effects of MVPA and TPA on protection from oxidative DNA damage. Increasing PA may mediate reduction in oxidative stress.

Keywords: Accelerometry; DNA damage; deoxyguanosine; exercise; oxidative stress.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Accelerometry
  • Adult
  • Aged
  • Alcohol Drinking / epidemiology
  • Body Mass Index
  • Carcinogenesis / metabolism
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Energy Intake
  • Exercise / physiology*
  • Female
  • Humans
  • Japan / epidemiology
  • Male
  • Middle Aged
  • Oxidative Stress*
  • Smoking / epidemiology
  • Surveys and Questionnaires

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine