Use of DMSO for the cryopreservation of Tritrichomonas foetus in liquid nitrogen

C M Campero

doi:10.1016/0304-4017(89)90083-6

Use of DMSO for the cryopreservation of Tritrichomonas foetus in liquid nitrogen

Vet Parasitol. 1989 Jun;31(3-4):339-43. doi: 10.1016/0304-4017(89)90083-6.

Author

C M Campero¹

Affiliation

¹ Departamento de Producción Animal EEA INTA Balcarce C.C., Buenos Aires, Argentina.

PMID: 2763452
DOI: 10.1016/0304-4017(89)90083-6

Abstract

Four Tritrichomonas foetus strains were successfully preserved in liquid nitrogen at -196 degrees C in the presence of 10% dimethylsulfoxide (DMSO) for 7 months (Strain 1) and 6 months (Strain 2, 3 and 4). The percentage of viable organisms after thawing ranged between 65 and 85%, depending upon the strain. The method used is described in detail.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Dimethyl Sulfoxide*
Freezing
Nitrogen*
Preservation, Biological*
Tritrichomonas / physiology*

Substances

Nitrogen
Dimethyl Sulfoxide