The objective of this work was to study the kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. The model terms glycerol concentration inoculum and C/N ratio with inoculum were found to be significant for lipid production. Lipid production was found to be higher in glycerol 82.5g/L, C/N ratio 75 and inoculum volume 6.25%. Optimized culture conditions were tested at 15L bench scale reactor. The biomass concentration and lipid content obtained was 29.5g/L and 50% (w/w), respectively. The yield coefficients were calculated and found to be 0.332g/g (g biomass/g of glycerol) of biomass and 0.179g/g (g lipid/g glycerol consumed) for lipid. Observed rates of lipid production show lipid production from 30h of fermentation. Out of the total glycerol consumed, 41.1% glycerol was converted into biomass, lipid, and citric acid.
Keywords: Biomass; Fermentation; Microbial lipids; RSM optimization; Yarrowia lipolytica.
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