Abstract
We have established transformed Chinese hamster ovary cells, which secrete hepatitis B virus surface antigen containing both pre-S1 and pre-S2 domains into culture medium, by using the autologous S gene promoter.
MeSH terms
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Animals
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Cells, Cultured
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Centrifugation, Density Gradient
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Cloning, Molecular
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DNA, Viral / genetics
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Electrophoresis, Polyacrylamide Gel
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Hepatitis B Surface Antigens / biosynthesis*
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Plasmids
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Protein Precursors / biosynthesis*
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Viral Envelope Proteins / biosynthesis*
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Viral Proteins / biosynthesis
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Viral Proteins / ultrastructure
Substances
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DNA, Viral
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Hepatitis B Surface Antigens
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Protein Precursors
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Viral Envelope Proteins
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Viral Proteins
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presurface protein 1, hepatitis B surface antigen
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presurface protein 2, hepatitis B surface antigen