Inotropic Effects of Prostacyclins on the Right Ventricle Are Abolished in Isolated Rat Hearts With Right-Ventricular Hypertrophy and Failure

J Cardiovasc Pharmacol. 2017 Jan;69(1):1-12. doi: 10.1097/FJC.0000000000000435.

Abstract

Background: Prostacyclin mimetics are vasodilatory agents used in the treatment of pulmonary arterial hypertension. The direct effects of prostanoids on right-ventricular (RV) function are unknown. We aimed to investigate the direct effects of prostacyclin mimetics on RV function in hearts with and without RV hypertrophy and failure.

Methods: Wistar rats were subjected to pulmonary trunk banding to induce compensated RV hypertrophy (n = 32) or manifest RV failure (n = 32). Rats without banding served as healthy controls (n = 30). The hearts were excised and perfused in a Langendorff system and subjected to iloprost, treprostinil, epoprostenol, or MRE-269 in increasing concentrations. The effect on RV function was evaluated using a balloon-tipped catheter inserted into the right ventricle.

Results: In control hearts, iloprost, treprostinil, and MRE-269 improved RV function. The effect was, however, absent in hearts with RV hypertrophy and failure. Treprostinil and MRE-269 even impaired RV function in hearts with manifest RV failure.

Conclusions: Iloprost, treprostinil, and MRE-269 improved RV function in the healthy rat heart. RV hypertrophy abolished the positive inotropic effect, and in the failing right ventricle, MRE-269 and treprostinil impaired RV function. This may be related to changes in prostanoid receptor expression and reduced coronary flow reserve in the hypertrophic and failing right ventricle.

MeSH terms

  • Animals
  • Cardiotonic Agents / pharmacology
  • Cardiotonic Agents / therapeutic use*
  • Heart Failure / drug therapy*
  • Heart Failure / physiopathology
  • Hypertrophy, Right Ventricular / drug therapy*
  • Hypertrophy, Right Ventricular / physiopathology
  • Male
  • Organ Culture Techniques
  • Prostaglandins I / pharmacology
  • Prostaglandins I / therapeutic use*
  • Rats
  • Rats, Wistar
  • Treatment Outcome
  • Vasodilator Agents / pharmacology
  • Vasodilator Agents / therapeutic use
  • Ventricular Function, Right / drug effects*
  • Ventricular Function, Right / physiology

Substances

  • Cardiotonic Agents
  • Prostaglandins I
  • Vasodilator Agents