Changes in surface capacitance and conductance parallel to phospholipid membranes associated with phase transition: effects of halothane

Biochim Biophys Acta. 1989 Sep 4;984(2):253-6. doi: 10.1016/0005-2736(89)90224-1.

Abstract

The effects of phase transition on the surface capacitance and conductance parallel to dipalmitoyl- (DPPC) and dimyristoyl-phosphatidylcholine (DMPC) membranes were studied by impedance dispersion. The phospholipid aggregates were embedded into pores of a polycarbonate filter and the impedance dispersions were measured at a frequency range from 30 Hz to 1.0 MHz. When the frequency was below 120 kHz, the capacitance showed a peak at the pretransition temperature and a steep rise at the main-transition temperature. In this system, the observed capacitance consists of frequency-dependent and -independent parts. The frequency-dependent part is a surface phenomenon and arises from the lateral motion of counterions at the membrane/water interface. The frequency-independent part represents mainly the properties of the bulk lipid phase. Addition of halothane decreased the total capacitance of the DPPC aggregates at the low frequency range to 1/2 to 1/8 of the control depending upon the temperature. The surface component was solely responsible for this capacitance decrease, because the non-surface component was slightly increased instead. The data suggest that halothane inhibited the lateral ionic flow parallel to the interface.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1,2-Dipalmitoylphosphatidylcholine*
  • Dimyristoylphosphatidylcholine*
  • Electric Conductivity
  • Halothane*
  • Liposomes*
  • Mathematics
  • Models, Theoretical
  • Molecular Conformation
  • Surface Properties
  • Thermodynamics

Substances

  • Liposomes
  • 1,2-Dipalmitoylphosphatidylcholine
  • Dimyristoylphosphatidylcholine
  • Halothane