Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production

Bo Yang; Haiqin Chen; Catherine Stanton; Yong Q Chen; Hao Zhang; Wei Chen

doi:10.1080/21655979.2016.1222996

Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production

Bioengineered. 2017 May 4;8(3):232-238. doi: 10.1080/21655979.2016.1222996. Epub 2016 Sep 22.

Authors

Bo Yang^{1

2}, Haiqin Chen¹, Catherine Stanton^{2

3}, Yong Q Chen¹, Hao Zhang^{1

2}, Wei Chen^{1

2

4}

Affiliations

¹ a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.
² b International Joint Research Laboratory for Probiotics at Jiangnan University , Wuxi , Jiangsu , P. R China.
³ c Teagasc Moorepark Food Research Center , Fermoy , Co. Cork , Ireland.
⁴ d Beijing Innovation Center of Food Nutrition and Human Health , Beijing Technology and Business University (BTBU) , Beijing , P.R. China.

Abstract

Conjugated linolenic acid (CLNA) is a family of isomers of linolenic acid with a number of health-associated benefits, which has been attracting great interest. Microbial CLNA producers are potentially an alternative source of CLNA for human nutrition. In present study, 16 neonate feces were collected and used for Bifidobacteria isolation, from which 25 bifidobacteria isolates were obtained. The bifidobacteria isolates were identified using 16s rDNA sequencing as Bifidobacterium adolescentis, B. breve, B. longum and B. pseudocatenulatum. These isolates were further investigated for their ability to produce CLNA using linolenic acid as substrate via GC-MS. The results showed most of the isolates could convert free linolenic acid into c9,t11,c15-CLNA and t9,t11,c15-CLNA at different levels. B. pseudocatenulatum was the most effective CLNA producer, which converted 86.91% of linolenic acid to c9,t11,c15-CLNA and 3.59% of to t9,t11,c15-CLNA isomer and the isolate exhibited to accumulate CLNA during 72 h culturing in which most CLNA isomers were in the supernatant fluid. The results indicated that utilization of this isolate for CLNA production will eliminate the purification process.

Keywords: bifidobacteria; bioconversion; conjugated linolenic acid; isolation.

MeSH terms

Bifidobacterium / classification
Bifidobacterium / isolation & purification*
Bifidobacterium / metabolism*
Feces / microbiology*
Female
Gastrointestinal Tract / microbiology*
Humans
Infant, Newborn
Linoleic Acids, Conjugated / biosynthesis*
Linoleic Acids, Conjugated / isolation & purification
Male
Species Specificity
Specimen Handling / methods*

Substances

Linoleic Acids, Conjugated

Grants and funding

This research was supported by the National Natural Science Foundation of China (Nos. 31571810, 31530056), the Program for New Century Excellent Talents (NCET-13-0831), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1249), the National Natural Science Foundation of Jiangsu Province (No. BK20150141), and the Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control.”