Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification

Marcos Vinicios Salles Dias; Vilma Regina Martins; Glaucia Noeli Maroso Hajj

doi:10.1007/978-1-4939-3804-9_11

Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification

Methods Mol Biol. 2016:1459:161-74. doi: 10.1007/978-1-4939-3804-9_11.

Authors

Marcos Vinicios Salles Dias¹, Vilma Regina Martins¹, Glaucia Noeli Maroso Hajj²

Affiliations

¹ International Research Center, AC Camargo Cancer Center, Rua Taguá 440, São Paulo, 01509-001, Brazil.
² International Research Center, AC Camargo Cancer Center, Rua Taguá 440, São Paulo, 01509-001, Brazil. [email protected].

PMID: 27665558
DOI: 10.1007/978-1-4939-3804-9_11

Abstract

This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.

Keywords: Extracellular vesicles; Gel chromatography; Nanoparticle tracking analysis (NTA); Stress-Inducible Protein 1 (STI1); Ultracentrifugation.

MeSH terms

Cell Fractionation
Chromatography, Gel
Enzyme-Linked Immunosorbent Assay
Extracellular Vesicles / metabolism*
Flow Cytometry
Heat-Shock Proteins / metabolism*
Protein Transport
Ultracentrifugation

Substances

Heat-Shock Proteins