The AMP-activated protein kinase beta 1 subunit modulates erythrocyte integrity

Exp Hematol. 2017 Jan:45:64-68.e5. doi: 10.1016/j.exphem.2016.09.006. Epub 2016 Sep 22.

Abstract

Failure to maintain a normal in vivo erythrocyte half-life results in the development of hemolytic anemia. Half-life is affected by numerous factors, including energy balance, electrolyte gradients, reactive oxygen species, and membrane plasticity. The heterotrimeric AMP-activated protein kinase (AMPK) is an evolutionarily conserved serine/threonine kinase that acts as a critical regulator of cellular energy balance. Previous roles for the alpha 1 and gamma 1 subunits in the control of erythrocyte survival have been reported. In the work described here, we studied the role of the beta 1 subunit in erythrocytes and observed microcytic anemia with compensatory extramedullary hematopoiesis together with splenomegaly and increased osmotic resistance.

MeSH terms

  • AMP-Activated Protein Kinases / genetics*
  • AMP-Activated Protein Kinases / metabolism*
  • Anemia, Hypochromic / genetics
  • Anemia, Hypochromic / metabolism
  • Anemia, Hypochromic / pathology
  • Animals
  • Erythrocytes / cytology
  • Erythrocytes / metabolism*
  • Erythrocytes / pathology
  • Erythrocytes / ultrastructure
  • Erythropoiesis / genetics
  • Female
  • Gene Expression
  • Male
  • Mice
  • Mice, Knockout
  • Protein Isoforms
  • Spleen / metabolism
  • Spleen / pathology

Substances

  • Protein Isoforms
  • Prkab1 protein, mouse
  • AMP-Activated Protein Kinases