Abstract
A 24-base oligonucleotide probe specific for a region of the Yersinia enterocolitica virulence plasmid (pYV) associated with HEp-2 cell cytotoxicity and the Sereny reaction was constructed by using sequences flanking critical TnphoA insertions in a subcloned fragment of pYV. This probe, highly specific and sensitive for virulent yersiniae, detected pathogenic Y. enterocolitica isolates in artificially inoculated foods.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Base Sequence
-
Cell Line
-
DNA Probes*
-
DNA, Bacterial / analysis
-
Food Microbiology*
-
Humans
-
Molecular Sequence Data
-
Nucleic Acid Hybridization
-
Plasmids
-
Predictive Value of Tests
-
Restriction Mapping
-
Sequence Homology, Nucleic Acid
-
Virulence
-
Yersinia enterocolitica / genetics
-
Yersinia enterocolitica / isolation & purification*
-
Yersinia enterocolitica / pathogenicity
Substances
-
DNA Probes
-
DNA, Bacterial