Development and substrate specificity screening of an in vivo biosensor for the detection of biomass derived aromatic chemical building blocks

Chem Commun (Camb). 2016 Sep 15;52(76):11402-11405. doi: 10.1039/c6cc04559f.

Abstract

Measuring substrate and/or product concentration can create a major bottleneck for synthetic and biosynthetic processes. Here we report the development and substrate screening of a whole cell biosensor to detect biomass-derived aromatic chemical building blocks, supporting the use of sustainable feedstocks in the bulk and fine chemical industries.

MeSH terms

  • Aldehyde Oxidoreductases / genetics
  • Aldehyde Oxidoreductases / metabolism
  • Benzene Derivatives / analysis*
  • Benzene Derivatives / metabolism
  • Biomass*
  • Biosensing Techniques / methods*
  • Coumaric Acids / analysis
  • Coumaric Acids / metabolism
  • Escherichia coli / metabolism
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Promoter Regions, Genetic
  • Substrate Specificity

Substances

  • Benzene Derivatives
  • Coumaric Acids
  • ferulic acid
  • Aldehyde Oxidoreductases