Abstract
MicroRNAs are short noncoding, ~22-nucleotide RNAs that regulate protein abundance. The growth in our understanding of this class of RNAs has been rapid since their discovery just over a decade ago. We now appreciate that miRNAs are deeply embedded within the genetic networks that control basic features of metazoan cells including their identity, metabolism, and reproduction. The Drosophila melanogaster model system has made and will continue to make important contributions to this analysis. Intended as an introductory overview, here we review the current methods and resources available for functional analysis of fly miRNAs for those interested in performing this type of analysis.
Keywords:
Drosophila melanogaster; miRNA; microRNA.
MeSH terms
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Animals
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / metabolism
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Gene Expression Profiling
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Gene Expression Regulation*
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Genes, Reporter
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Genetic Techniques
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / classification
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Nucleic Acid Conformation
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Oligonucleotide Array Sequence Analysis
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RNA Isoforms / antagonists & inhibitors
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RNA Isoforms / genetics
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RNA Isoforms / metabolism
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / metabolism
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Ribonuclease III / genetics*
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Ribonuclease III / metabolism
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Signal Transduction
Substances
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Drosophila Proteins
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MicroRNAs
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Pasha protein, Drosophila
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RNA Isoforms
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RNA, Small Interfering
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RNA-Binding Proteins
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Ribonuclease III
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drosha protein, Drosophila