Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis

Molecules. 2016 Oct 9;21(10):1335. doi: 10.3390/molecules21101335.

Abstract

The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n = 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 10⁴ g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC50 0.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC50 0.29 mg/mL), and hydroxyl radical scavenging activity (IC50 0.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant.

Keywords: Semen Juglandis; antioxidant activities; physicochemical property; polysaccharide; response surface methodology (RSM).

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology
  • Arabinose / chemistry
  • Arabinose / isolation & purification
  • Free Radical Scavengers / chemistry*
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / pharmacology
  • Fucose / chemistry
  • Fucose / isolation & purification
  • Galactose / chemistry
  • Galactose / isolation & purification
  • Hexuronic Acids / chemistry
  • Hexuronic Acids / isolation & purification
  • Juglans / chemistry*
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology
  • Rhamnose / chemistry
  • Rhamnose / isolation & purification

Substances

  • Antioxidants
  • Free Radical Scavengers
  • Hexuronic Acids
  • Polysaccharides
  • Fucose
  • galacturonic acid
  • Arabinose
  • Rhamnose
  • Galactose