Abstract
Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.
Keywords:
Haploid post-meiotic spermatid; Male germ cell; Mouse testis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Separation / methods*
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Centrifugation, Density Gradient
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Chromatin Immunoprecipitation
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Epigenesis, Genetic*
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Fluorocarbons / chemistry
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Histone Deacetylase Inhibitors / pharmacology
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Histone Deacetylases / genetics*
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Histone Deacetylases / metabolism
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Histones / genetics*
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Histones / metabolism
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Hydroxamic Acids / pharmacology
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Male
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Meiosis
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Mice
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Serum Albumin, Bovine / chemistry
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Spermatids / cytology*
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Spermatids / metabolism
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Spermatocytes / cytology*
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Spermatocytes / metabolism
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Spermatogenesis / genetics
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Testis / cytology
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Testis / growth & development
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Testis / metabolism
Substances
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Fluorocarbons
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Histone Deacetylase Inhibitors
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Histones
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Hydroxamic Acids
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fluorocarbon 77
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Serum Albumin, Bovine
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trichostatin A
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Histone Deacetylases