Abstract
Choisya 'Aztec-Pearl', a hybrid of Choisya ternata and Choisya dumosa var. arizonica, had the antinociceptive activity in the ethanol extract (EECA) of its leaves evaluated. Two quinoline alkaloids, anhydroevoxine (A) and choisyine (C), isolated from these leaves were also tested. The results obtained pointed out to a very high antinociceptive activity measured by the hot plate model for EECA (at doses of 10, 30 and 100 mg/kg) as well as for A and C (at doses of 1, 3 and 10 mg/kg). The magnitude of the activity was two-fold higher than the one observed for the morphine treated animals for the higher doses of extracts/compounds (30, 100 mg/kg and 3, 10 mg/kg respectively). The mechanism of action for this activity was also investigated and it seems that for EECA as well as A and C, the opiate system plays an important role. Results have also shown that the nitric oxide (NO) system also play a pivotal role in the case of EECA and A while for C it seems that the cholinergic system have some involvement. The acute toxicity was evaluated for EECA with results showing no important toxic effect.
MeSH terms
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Alkaloids / adverse effects
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Alkaloids / isolation & purification*
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Alkaloids / pharmacology
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Analgesics / adverse effects
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Analgesics / pharmacology*
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Animals
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Male
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Mice
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Plant Extracts / adverse effects
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Plant Extracts / pharmacology*
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Quinolines / adverse effects
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Quinolines / isolation & purification*
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Quinolines / pharmacology
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Rutaceae / chemistry*
Substances
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Alkaloids
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Analgesics
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Plant Extracts
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Quinolines
Grants and funding
This work was financed by the following granting agencies: SFI (Science Foundation Ireland), CAPES (Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior), FAPERJ (Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), the High Education Authority’s Programme for Research in Third- Level Institutions Cycle 5’s funding support for TBSI. SFI programme ISCA-Brazil Grant no. SFI/13/ISCA/2843, CAPES Grant no BEX 5962 13-7, FAPERJ Grant no E26/203.018/2015, CNPq Grant no 401683/2013-3. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.