Preventive effects of blueberry extract on behavioral and biochemical dysfunctions in rats submitted to a model of manic behavior induced by ketamine

Brain Res Bull. 2016 Oct:127:260-269. doi: 10.1016/j.brainresbull.2016.10.008. Epub 2016 Oct 18.

Abstract

The aim of the present study was to evaluate the protective effects of blueberry extract on oxidative stress and inflammatory parameters in a model of mania induced by ketamine administration in rats. Male rats were pretreated with blueberry extract (200mg/kg, once a day for 14days), lithium chloride (45mg/kg, mood stabilizer used as a positive control, twice a day for 14days), or vehicle. Between the 8th and 14th days, rats also received an injection of ketamine (25mg/kg) or vehicle. In the 15th day, thirty minutes after ketamine administration the hyperlocomotion of the animals was assessed in the open - field apparatus. Immediately after the behavioral analysis brain and blood were collected for biochemical determinations. ketamine treatment induced hyperlocomotion and oxidative damage in cerebral cortex, hippocampus and striatum such as an increase in lipid peroxidation and a decrease in the antioxidant enzymes activities (superoxide dismutase, catalase e glutatione peroxidase). Ketamine administration also increased the IL-6 levels in serum in rats. Pretreatment of rats with blueberry extract or lithium prevented the hyperlocomotion, pro - oxidant effects and inflammation induced by ketamine. Our findings suggest that blueberry consumption has a neuroprotective potential against behavioral and biochemical dysfunctions induced in a preclinical model that mimic some aspects of the manic behavior.

Keywords: Blueberry; Cytokines; Hyperlocomotion; Ketamine; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bipolar Disorder / drug therapy*
  • Bipolar Disorder / metabolism
  • Bipolar Disorder / pathology
  • Blueberry Plants*
  • Brain / drug effects
  • Brain / metabolism
  • Brain / pathology
  • Catalase / metabolism
  • Disease Models, Animal
  • Glutathione Peroxidase / metabolism
  • Interleukin-6 / blood
  • Ketamine
  • Lithium Compounds / pharmacology
  • Male
  • Motor Activity / drug effects
  • Motor Activity / physiology
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Phytotherapy*
  • Plant Extracts / pharmacology*
  • Psychotropic Drugs / pharmacology*
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism

Substances

  • Interleukin-6
  • Lithium Compounds
  • Plant Extracts
  • Psychotropic Drugs
  • Thiobarbituric Acid Reactive Substances
  • Ketamine
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase