Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles

X R Zhang; Y Zhang; F T Chen; Y Li; S S Zhang

doi:10.1039/c6cc06735b

Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles

Chem Commun (Camb). 2016 Nov 3;52(90):13261-13264. doi: 10.1039/c6cc06735b.

Authors

X R Zhang¹, Y Zhang¹, F T Chen¹, Y Li¹, S S Zhang²

Affiliations

¹ Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
² Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, P. R. China. [email protected].

PMID: 27775109
DOI: 10.1039/c6cc06735b

Abstract

A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs). As a proof-of-principle, novel applications of this method as a versatile biosensor for single-nucleotide polymorphisms (SNPs) and DNA methyltransferase (MTase) were presented.

MeSH terms

Biosensing Techniques / methods*
Calorimetry / methods*
Click Chemistry
Copper / chemistry*
DNA Modification Methylases / metabolism*
Gold / chemistry*
Ion Exchange
Metal Nanoparticles / chemistry*
Polymorphism, Single Nucleotide*

Substances

Gold
Copper
DNA Modification Methylases
cupric sulfide