Abstract
Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.
MeSH terms
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Animals
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COS Cells
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Cell Culture Techniques
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Cell Line, Tumor
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Cell Membrane Permeability
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Embryonic Stem Cells
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Fluorescent Dyes / radiation effects
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Humans
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Ligands
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Light
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Mice
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Microscopy, Fluorescence
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Molecular Structure
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Photochemical Processes*
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Photochemistry / methods
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Recombinant Fusion Proteins / metabolism
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Single Molecule Imaging / methods*
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Small Molecule Libraries / chemical synthesis*
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Small Molecule Libraries / chemistry*
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Small Molecule Libraries / metabolism
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Small Molecule Libraries / radiation effects
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Spectrometry, Fluorescence
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Spectrophotometry, Ultraviolet
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Staining and Labeling
Substances
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Fluorescent Dyes
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Ligands
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Recombinant Fusion Proteins
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Small Molecule Libraries