Hodgkin lymphoma, HIV, and Epstein-Barr virus in Malawi: Longitudinal results from the Kamuzu Central Hospital Lymphoma study

Pediatr Blood Cancer. 2017 May;64(5):10.1002/pbc.26302. doi: 10.1002/pbc.26302. Epub 2016 Oct 26.

Abstract

Background: Contemporary descriptions of classical Hodgkin lymphoma (cHL) are lacking from sub-Saharan Africa where human immunodeficiency virus (HIV) and Epstein-Barr virus (EBV) are prevalent.

Methods: We describe a prospective cHL cohort in Malawi enrolled from 2013 to 2015. Patients received standardized treatment and evaluation, including HIV status and EBV testing of tumors and plasma.

Results: Among 31 patients with confirmed cHL, the median age was 19 years (range, 2-51 years) and 22 (71%) were male. Sixteen patients (52%) had stage III/IV, 25 (81%) B symptoms, and 16 (52%) performance status impairment. Twenty-three patients (74%) had symptoms >6 months, and 11 of 29 (38%) had received empiric antituberculosis treatment. Anemia was common with median hemoglobin 8.2 g/dL (range, 3.1-17.1 g/dL), which improved during treatment. No children and 5 of 15 adults (33%) were HIV+. All HIV+ patients were on antiretroviral therapy for a median 15 months (range, 2-137 months), with median CD4 count 138 cells/μL (range, 23-329 cells/μL) and four (80%) having undetectable HIV. EBV was present in 18 of 24 (75%) tumor specimens, including 14 of 20 (70%) HIV- and 4 of 4 (100%) HIV+. Baseline plasma EBV DNA was detected in 25 of 28 (89%) patients, with median viral load 4.7 (range, 2.0-6.7) log10 copies/mL, and subsequently declined in most patients. At 12 months, overall survival was 75% (95% confidence interval [CI], 55%-88%) and progression-free survival 65% (95% CI, 42%-81%). Baseline plasma EBV DNA and persistent viremia during treatment were associated with poorer outcomes.

Conclusion: cHL in Malawi is characterized by delayed diagnosis and advanced disease. Most cases were EBV associated and one-third of adults were HIV+. Despite resource limitations, 12-month outcomes were good.

Keywords: Epstein-Barr virus; Hodgkin lymphoma; Malawi; pediatric cancer; sub-Saharan Africa.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • CD4 Lymphocyte Count
  • Child
  • Child, Preschool
  • DNA, Viral / blood
  • Disease-Free Survival
  • Epstein-Barr Virus Infections / complications
  • Epstein-Barr Virus Infections / epidemiology*
  • Female
  • HIV Infections / complications
  • HIV Infections / epidemiology*
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / isolation & purification*
  • Hodgkin Disease / complications
  • Hodgkin Disease / epidemiology*
  • Humans
  • Longitudinal Studies
  • Malawi / epidemiology
  • Male
  • Middle Aged
  • Prospective Studies
  • Risk Factors
  • Treatment Outcome
  • Viral Load
  • Viremia / virology
  • Young Adult

Substances

  • DNA, Viral