Capping of HLA antigens in human lymphocytes as followed by immunogold label-fracture

J Histochem Cytochem. 1989 Oct;37(10):1489-96. doi: 10.1177/37.10.2778307.

Abstract

We used immunogold label-fracture to follow the migration of HLA I class and HLA II class antigens during capping as induced by specific monoclonal antibodies. Capping is achieved through a process of clustering and "consolidation" of clusters into larger patches and, finally, a single cap. All receptors appear to cluster from the very start, with no "stray" molecules joining already formed patches. Characterization of exoplasmic and protoplasmic fracture-faces of capping cells fails to reveal any corresponding accumulation of intramembrane particles and/or subtler rugosities. Our results are consistent with the concepts that view the migration of capping molecules as contemporaneous with the efflux of noncapping integral membrane proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Freeze Fracturing
  • Histocompatibility Antigens Class I / analysis
  • Histocompatibility Antigens Class I / immunology*
  • Histocompatibility Antigens Class II / analysis
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Immunohistochemistry
  • Immunologic Capping*
  • Lymphocytes / immunology*
  • Lymphocytes / ultrastructure
  • Microscopy, Electron

Substances

  • Antibodies, Monoclonal
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II