Two better cell lines for making hybridomas expressing specific T cell receptors

J Immunol. 1989 Sep 15;143(6):1822-5.

Abstract

Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Fusion* / methods
  • Cell Line
  • Chromosome Deletion
  • Hybridomas / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Receptors, Antigen, T-Cell / biosynthesis
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / isolation & purification*
  • Thymoma / metabolism
  • Tumor Cells, Cultured / metabolism

Substances

  • Receptors, Antigen, T-Cell

Associated data

  • GENBANK/M29272