NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues

Cuiwen He; Loren G Fong; Stephen G Young; Haibo Jiang

doi:10.1136/jim-2016-000239

NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues

J Investig Med. 2017 Mar;65(3):669-672. doi: 10.1136/jim-2016-000239. Epub 2016 Oct 28.

Authors

Cuiwen He¹, Loren G Fong¹, Stephen G Young^{1

2}, Haibo Jiang³

Affiliations

¹ Departments of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
² Departments of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
³ Centre for Microscopy, Characterisation, and Analysis, The University of Western Australia, Perth, Western Australia, Australia.

Abstract

Over the past few decades, several approaches have been used to image lipids in cells and tissues, but most have limited spatial resolution and sensitivity. Here, we discuss a relatively new approach, nanoscale secondary ion mass spectrometry imaging, that makes it possible to visualize lipids in cells and tissues in a quantitative fashion and with high spatial resolution and high sensitivity.

Keywords: Lipids.

Publication types

Review
Research Support, N.I.H., Extramural

MeSH terms

Animals
Cells / metabolism*
Humans
Lipids / analysis*
Nanotechnology / methods*
Organ Specificity*
Spectrometry, Mass, Secondary Ion / methods*

Substances

Lipids

Abstract

Publication types

MeSH terms

Substances

Grants and funding